Abstract:
Diabetes mellitus type 2 (DMT2) is a disorder characterized by hyperglycemia due to impaired insulin secretion and insulin resistance. The purpose of this study was to identify the gene involved in the positive allele of DMT2 marker using A18 primer and is a type of exploratory descriptive study. The samples used were 2 samples of DMT2 patients from the villages of Taman and Balongbend, and 8 samples of DNA collection at the Molecular Biology Laboratory, Muhammadiyah University of Sidoarj. Based on the genetic analysis carried out, the GATB gene (Glutamyl-tRNA Amidotransferase Sub Unit B) was identified in the positive allele with a homology value of "percentage identity" between the sample sequence and the sequence data in the NCBI Genbank of 80 and 88%. This research hypothesizes that the GATB gene has a relationship with the emergence of DMT2 as evidenced by the relationship between the gene and the performance of mitochondria to produce ATP which is needed by pancreatic beta cells to produce insulin.
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References
Internasional Diabetes Federation. (2019). IDF Diabetes Atlas Ninth Edition 2019. Belgia : Avenue Herrmann-Debroux 54 B-1160 Brussels
Zahid, R.A., Bilal, K. M., Baydaa, A.Abd. (2011). Molecular Investigation of Genetic Polymorphisms in Type 2 Diabetic Patients Usng Random Amplified Polymorphic DNA (RAPD-PCR). Iraqi Journal of Cancer and Medical Genetics, 4(2), 47-5. Retrieved from https://www.iasj.net/iasj?func=fulltext&aId=56172
Sari, F. K. (2020). Analisa Polimorfisme Pada Penderita Diabetes Melitus Tipe 2 Menggunakan Metode Random Amplified Polimorphic DNA Berdasarkan Primer R4, D20 dan A18 (Undergraduate’s thesis). Universitas Muhammadiyah Sidoarjo
Suparnintyas J. F., Okky D. P., Devit P., Teuku T. (2008). Phylogenetic Analysis of Rubber Tree Clones Using AFLP (Amplified Fragment Length Polymorphism) Marker. Jurnal Bioteknologi dan Biosains Indonesia (JBBI), 5(1): 8. http://doi.org/10.29122/jbbi.v5il.2544
Septiaputri A. A., Giri R. B., Muh. Alsere B. S., Rahma D. A., Nadia F., … ., Asadatuun A. (2020). Comparison of DNA Isolation Methods For Fresh and Processed Seafood. Indonesian Fiseries Processing Journal, 23(3). https://doi.org/10.17844/jphpi.v23i3.32314
Healey A., Agnelo F., Tal C., Robert J.H. (2014). Protocol : A Simple Method for extracting Next- Generation Sequencing Quality Genomic DNA From Recalcitrant Plant Species. Plant Methods, 10(21).https://doi.org/10.1186/1746-4811-10-21
Bagus I. W., Wirawan P. G. I., Adiartayasa W.I. (2019).Analisis Homologi Fragmen DNA CVPD dari Jeruk Kinkit Trophasia trifolia Menggunakan BLAST Protein Dan BLAST Nukleotida. Jurnal Agroteknologi Tropika, Vol 8 No. 4, 381-387, Retrieved from https://ojs.unud.ac.id/index.php/JAT/article/view/54479
Suyono, yoyon. (2010). Determination of Pseudomonas Bacteria Spesies and Analysis of Phylogenetic Tree using Bioinformatics Method.Jurnal Biopropal Indonesia, Vol 9 No.2, Retrieved from http://lib.kemenperin.go.id/neo/detail.php?id=178667
Ihsan N.Y., Fellatami K., Permana R., Mulyani Y., Pribadi K. D.T. (2020). Analysis of Bacteria in The Reduction of The Concentration of Lead Metal Pb(CH3COO)2 Using Gen 16S Rrna. Indonesian Journal of Merine Science and Technology, Vol 13 No.2, 2476-9991. https://doi.org/10.21107/jk.v13i2.7285
Nagao, A., Suzuki, T., Katoh, T., Sakaguchi, Y., Suzuki, T., (2009). Biogenesis of Glutaminyl-mt tRNA Gln in Human Mitochondria. Proceedings of the National Academy os Sciences of the United States of America, Vol 106 No.38 113-8656. https://doi.org/10.1073/pnas.0907602106
Webb, D. B., Diaz, A. G., Prasun, P., (2020). Mitochondrial Translation Defect and Human Disease. National Library of Medicine NCBI, Vol 4, 71-80, https://doi.org/10.20517/jtgg.2020.11
Friederich, W. M., Timal, S., Powell, A. C., Dallabona, C., Kurolap, Zambrano, P.S. (2018). Pathogenic Variants in Glutamyl-tRNA Gln Amidotransferase subunits Cause a Lethal Mitochondrial Cardiomypathy Disorder. National Library of Medicine, Vol 9 No.1. https://doi.org/10.1038/s41467-018-06250-w
Yuliana, Dewi. (2012). Kajian Mutasi Gen Pada DNA Mitokondria (mtDNA) Sebagai Predisposisi Diabetes Mellitus. As-Syifaa Jurnal Farmasi. Vol 4 N0 1, Retrieved from https://jurnal.farmasi.umi.ac.id/index.php/as-syifaa/article/view/146
Azizah, I.M. (2020). Penyakit Mitokndria: Review. Research Gate Universitas Padjajaran. Retrieved from https://www.researchgate.net/publication/346678225_Penyakit_Mitokondria_Review